by Love 'n Let Aviary on Sat Jul 14, 2007 9:06 am
Yes, they are different species, so should not be encouraged to breed together even if they are a male/female pair.
However, just this behavior that you described alone (one bird threatening another from inside the nestbox) does not mean that both birds are females. A female will do this to a male as well, if she decides that she wants to go to nest but doesn't particularly like him enough to accept him as a mating partner. The bird that is doing the threatening from inside the nestbox is claiming that nestbox as a nesting site and doesn't accept that other bird as a parenting partner, regardless of the sex of each of the birds.
If two females (or two males) get along well enough on the personality level, they can both "nest" in the same nestbox without any fighting or arguing. There are many people who have found 10 or more eggs in their nestbox (indicating two females instead of a male and a female).
The only way of knowing the sex of your lovebirds for sure is if either of them lays an egg (definitely a female) or you have them DNA or surgically sexed.